Botox Training in Charlotte (Mooresville, NC) — CME-Accredited Small Group Certification

Dual-MD Clinical Training in North Carolina

Charlotte / Mooresville Botox and Dermal Filler Training for 2027: Two-MD Clinical Site

ACCME triple-accredited for 2027. Taught at a dedicated clinical training site in Mooresville, North Carolina by Dr. Andy Castillo and Dr. Rebecca Appleton Castillo, both MDs licensed in North Carolina and co-directors of the Charlotte Training Center. 11.75 total contact hours including 4.7 nursing pharmacology hours. Maximum 4 attendees per session, with guaranteed 20u of BTX-A and 1cc of HA filler included for live hands-on practice.

11.75 Total Contact Hours
7.25 Online CME Hours (self-paced, received the day of registration, 2-year access with updates) plus a separate 4.5-Hour Live Hands-On Certificate of Completion.
Book Regenerative Aesthetics Training (PRP, microneedling) the same Charlotte weekend for 23 Total Hours and the PRP Facelift protocol unlock.
4.7 Nursing Pharmacology Hours Included. Direct ACCME accreditation is what allows pharmacology hours to be awarded. Programs routed through AAFP, dental boards, or state society "equivalence" credit cannot award pharmacology hours. Rolled out in 2026 after attendee feedback.
ACCME Accredited AMA Category 1 AANP CEU + Pharma ANCC CEU + Pharma
Gregory P. Zengo, MD, FACOG

Gregory P. Zengo, MD, FACOG

Founder & Medical Director, PracticalCME. Author of the Botox and Dermal Filler curriculum (2012). Carruthers-trained 2001. 24 years of injection experience.

Alissandro Castillo, MD

Alissandro "Andy" Castillo, MD

Co-Director, Charlotte / Mooresville Clinical Training Site. State-licensed in North Carolina.

Rebecca Appleton Castillo, MD

Rebecca Appleton Castillo, MD

Co-Director, Charlotte / Mooresville Clinical Training Site. State-licensed in North Carolina.

The Charlotte Botox and Dermal Filler session is taught at a dedicated clinical training site in Mooresville, North Carolina (Lake Norman area, approximately 30 miles north of downtown Charlotte), inside Dr. Andy Castillo and Dr. Rebecca Appleton Castillo's shared practice. Both Castillos co-teach the course, which gives attendees two licensed MDs in the room for direct supervision throughout the hands-on. The venue is accessible from Charlotte Douglas International Airport (CLT) and serves aesthetic clinicians across North Carolina, South Carolina, and the broader Carolinas and Southeast. The curriculum covers the three FDA-labeled toxin indications plus the 2024 FDA approval for platysma bands, dosing and handling for all 6 FDA-approved neurotoxin brands, and the off-label indications well-supported in the peer-reviewed literature. Dermal filler hands-on covers lips, perioral lines, nasolabial folds, and marionette lines. For the full positioning, see the Botox and Filler training hub page.

Why Charlotte-Area Clinicians Enroll with PracticalCME

  • Clinical Training Site in Mooresville, NC. Dedicated medical facility, not a hotel conference room.
  • Two MDs On-Site. Both Dr. Castillos co-teach, licensed in North Carolina.
  • Continuous Curriculum Since 2012. Fourteen years of revision, independent of any manufacturer.
  • 4.7 Nursing Pharmacology Hours. Direct accreditation, not third-party equivalence.
  • Open to RN, NP, PA, MD, DO, DDS. Where NC or SC state scope allows.
  • Max 4 Attendees per Session. Direct one-on-one supervision with two MDs in the room.
  • Guaranteed Product Included. 20u BTX-A and 1cc HA filler for every attendee.
  • No Cap on Hands-On. Bring your own patients, purchase additional product at below-retail.
  • Patient Consented by PCME MD. You never pay or collect from the patient.
  • No Level 2 Class. Full-face toxin and standard filler indications in one course.
  • Online Course Delivered Day of Registration. 2 years of access with free updates.
  • Affirm Financing. 0% APR for 6 months available. No sales call.
  • 2027 A-T.com Buyer's Guide Member. Meets or Exceeds all 8 quality criteria for inclusion. Read the review.
Book Regenerative Aesthetics on the Same Charlotte Weekend: Pair with the PracticalCME Regenerative Aesthetics course (PRP, microneedling, topical exosomes, red LED) for 23 total contact hours and the full PRP Facelift protocol integrating platelet-rich plasma, dermal filler, and botulinum toxin.
CME Credit: 7.25 AMA PRA Category 1 Credits (physicians and PAs via AAPA reciprocity), 7.25 AANP contact hours including 4.7 pharmacology hours (NPs), 7.25 ANCC contact hours including 4.7 pharmacology hours (RNs), plus a separate 4.5-hour Hands-On Certificate of Completion for the live Charlotte / Mooresville session. Accredited through the joint providership of Global Education Group (Global) and PracticalCME, LLC. Global is accredited by the ACCME to provide continuing medical education for physicians.

0% Financing: Available at checkout through our partner Affirm. Terms subject to Affirm approval.
Triple Accreditation: AMA Category 1, AANP, and ANCC 2027 A-T.com Buyer's Guide Member

📍🗓️🛒Charlotte Course Location, Dates, and Registration


🗓️Saturday November 14, 2026

⚠️3 Regen Aesthetic Seats Left

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🗓️Saturday January 16, 2027

✅ Seats Available

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🗓️Saturday March 13, 2027

✅ Seats Available

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🗓️Saturday August 28, 2027

🔜 Contact info@practicalcme.com to reserve this date early

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🗓️Saturday October 16, 2027

🔜 Contact info@practicalcme.com to reserve this date early

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🗓️Saturday December 4, 2027

🔜 Contact info@practicalcme.com to reserve this date early

 

Hands-On Class Times

🕗8 am-Noon Botox & Filler

🗺️ PracticalCME Hands-On Training Center, Mooresville, NC

Click Map for Location and Directions

📃Charlotte Botox & Dermal Filler Course Outline

🖥️ All Courses Feature Blended Online + Live FAST TRACK Training
💉20u BTX-A and 1cc Hyaluronic Acid Dermal Filler Included for Hands-On
          Additional Hands-On Can Be Performed Upon Request
👨‍⚕️One-On-One MD Supervised Hands-On (Limit 5 per Class)
🎗️7.25 AMA, AANP, or ANCC CME/CEU Credit Hours, and 4+ ANCC, AANP Pharmacology Credits for the online training module, plus a second 4.5 Hour Hands-On Certificate of Completion.
Online Access for 2 Years and Free Updates
 

STEP 1: Botulinum Toxins and Dermal Fillers in Aesthetic Medicine Online
Course Outline

  1. Introduction to Injectable Aesthetic Medicine

    • Overview of neuromodulator and dermal filler procedures, the post-graduate training landscape, and the provider vs. injector philosophy
  2. Facial Aging and Wrinkle Formation

    • Mechanisms of dynamic and static wrinkle development including muscle activity, dermal structural loss, and fat pad displacement
  3. Skin Anatomy and the Psychology of the Face

    • Layered skin structure relevant to injection depth, and the psychological and social impact of facial wrinkles on patient communication
  4. Botulinum Toxin Pharmacology and FDA-Approved Products

    • Mechanism of action, comparison of all six approved neuromodulator formulations, reconstitution, and safe product sourcing
      • Botox®
      • Dysport®
      • Xeomin®
      • Jeuveau®
      • Daxxify®
      • Letybo®
  5. Facial Musculature for Neuromodulator Treatment

    • Identification of upper and lower face muscles, wrinkle patterns, anatomical danger zones, and the basis for treatment design
  6. Patient Selection, Consent, and Pre-Treatment Assessment

    • Candidate criteria, contraindications, movement assessment, baseline photography, and recognition of pre-existing ptosis
  7. Neuromodulator Treatment Design: Five-Star Indications

    • Evidence-based dose selection and individualized treatment design for Glabellar Lines, Crow’s Feet, and Forehead Lines, including conservative dosing strategy and lowest effective dose principles
  8. Neuromodulator Treatment Design: Additional Indications

    • Lower face and neck applications, clinical candidacy criteria, and identifying when treatment should be declined based on risk-benefit and patient selection
    • Platysma Bands, Brow Lift, DAO Lip Corners, Upper Lip “Lip Flip”, Chin Dimpling, Gummy Smile, Bunny Lines, Facial Slimming.
  9. Injection Technique, Post-Treatment Care, and Complication Management

    • Technique principles, post-injection protocols, managing uneven results, bruising, lid ptosis, and early wear-off
  10. Dermal Filler Pharmacology, Product Selection, and Clinical Rationale

    • Overview of all FDA-approved dermal filler categories; evidence-based rationale for focusing clinical training on hyaluronic acid fillers based on superior safety profile, patient satisfaction, and reversibility compared to permanent and semi-permanent alternatives
  11. Filler Patient Selection, Consent, and Safety Principles

    • Candidate criteria, contraindications, vascular anatomy awareness, low-pressure injection technique, and aspiration considerations
  12. Filler Treatment Design:  FDA-Approved Linear Folds and Peri-Oral Applications

    • Treatment design for Nasolabial Folds, Marionette Lines, and lip augmentation with emphasis on conservative volume selection
    • Complete instruction on Lip Design (border and body injection), Ethnicity differences and intra-arterial injection prevention.  NO “ADVANCED” COURSE NEEDED.
  13. Filler Complication Recognition and Management

    • Early recognition of vascular occlusion and tissue ischemia, immediate management steps, and hyaluronidase use
  14. Practice Integration and Treatment Planning

    • Building a treatment plan that combines toxin and filler, managing patient expectations, and designing treatments that prioritize long-term patient retention over upselling

STEP 2: Small Group Hands-On Training (both sessions on the same date)

Botulinum Toxin A Training Hands-On Certification (LIVE Small Group in Charlotte-Mooresville, NC)

  • LIVE HANDS-ON INJECTION opportunity for qualified licensed attendees (RN, NP, PA, MD, DO, DDS)
    • Course Review and Q&A with our Experienced MD Faculty
    • Pre-select a friend or relative to be your hands-on patient based on your knowledge from the online materials already completed.
    • One-On-One Supervised hands-on with our faculty of that patient you provide.  From consent to treatment design, to injections to post-treatment instructions.
    • 20u BTX-A and all supplies are provided in your tuition.  Additional BTX-A hands-on can be performed upon request.

Charlotte Hyaluronic Acid Dermal Filler Hands-On Certification (LIVE Small Group in Charlotte-Mooresville, NC)

  • LIVE HANDS-ON INJECTION opportunity for qualified licensed attendees (RN, NP, PA, MD, DO, DDS)
    • Course Review and Q&A with our Experienced MD Faculty
    • Pre-select a friend or relative to be your hands-on patient based on your knowledge from the online materials already completed.
    • One-On-One Supervised hands-on with our faculty on that patient you provide.  Up to 1cc Hyaluronic Acid Filler and all supplies are included in your tuition.  Additional HA Filler Hands-On can be performed upon request.

Your instructors in Charlotte rebecca-castille andy-castillo for hands-on at our clinical training site in Mooresville, NC are Andy Castillo, MD and Rebecca Castillo, MD. 
They have over 15 years of experience performing Botox and Filler Injections and have trained for PracticalCME for over 7 years, teach at Duke University and have even earned a patent.
They have built a successful ⭐⭐⭐⭐⭐ practice from scratch and incorporate the best of all aesthetic modalities  under one roof.  They have extensive experience training RN’s and NP’s who have gone on to full, successful aesthetic careers in their own practice and throughout the country.  In 2026, Andy was elected to the prestigious ASLMS Laser Surgery Board of Directors.

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The instructor for your Online portion is gregory-p-zengo-medical-director-practicalcme Gregory Zengo, MD who has injected over 1.3 million units of Botox and has done Dermal Filler Injections for 22 years. A native of Rockland County, NY, he attended medical school and residency in Atlanta where he was Chief Resident and elected Resident Teacher of the Year before joining the faculty at Emory University School of Medicine. He has been a medical director of 3 different medspas and has hired and worked with numerous NP’s and RN’s in aesthetics outside of PracticalCME.  As the lead medical director of PracticalCME since 2008, he remains in charge of CME accreditation and the online curriculum in addition to live hands-on at 4 sites. Finally, Dr. Zengo also authored the world’s first CME-accredited courses on PRP and Microneedling in 2014, and PDO Threads in 2018.

Once you register, you will be encouraged to contact any of your physician faculty members with any questions you might have before, during and after your training is complete.

ACCME Accredited PracticalCME Training
ACCME (AMA Cat. 1) Accredited Training
PracticalCME is ANCC Accredited
ANCC Accredited Training
Practical CME awards AANP credits
AANP Accredited Training
PracticalCME Joint CME Provider
CME Joint Provider

This Activity Consists of Two Distinct Components…

1. Botulinum Toxins and Dermal Fillers in Aesthetic Medicine … Streaming Online Course (7.25 CME/CEU Hours)

This activity has been planned and implemented in accordance with the accreditation requirements and policies of the Accreditation Council for Continuing Medical Education (ACCME) through the joint providership of Global Education Group (Global) and PracticalCME, LLC.  Global is accredited by the ACCME to provide continuing medical education for physicians.  See the Full Accreditation Statement

Physicians: 7.25 AMA PRA Category 1 Credits™
PA’s: 7.25 AMA PRA Category 1 Credits™ (Reciprocity with AAPA)
NP’s: AANP 7.25 contact hours (which includes 4.7 hours of pharmacology)
RN’s: ANCC 7.25 contact hours including 4.75 pharmacotherapeutic contact hours
Other providers will receive a 7.25 hour AMA Certificate for submission to your licensing board for equivalency credit.

2. Botulinum Toxins and Dermal Fillers Small Group Live Hands-On Training (Up to 4.5 Additional Supervised Hands-On non-CME Contact Hours)

This live activity will offer live review, Q&A, Patient Assessment, Consent and MD-Supervised Hands-On Treatment of cosmetic Botulinum Toxin and HA Dermal Filler treatment indications.  Attendees provide a suitable patient based on your knowledge learned from the online certification course.  The 20u of BTX-A and up to 1cc of HA Dermal Filler for your supervised hands-on is included in your course tuition.  Additional toxin and filler for extended hands-on practice can be purchased.  Maximum class size is 5 attendees per MD hands-on instructor.

A 4.5-Hour Hands-On Certificate of Completion will be presented upon completion of this course for qualification for professional liability insurance and credentialing.

 

First, Reserve your spot in one of our blended FAST TRACK Training Courses for the Date and Location of your Choice.

online-video-cme-courses

Then Watch the Online training at your own pace.  Follow clickable links and interact with our faculty for any questions you have.  When finished, complete the online post-test.

DOWNLOAD/PRINT CME CERTIFICATE #1

 

AMA Category 1 Certification Credits for this Training Course

Finally, Attend the Live Hands-On Date and Complete Your Small-Group Hands-On Training in Botox and Dermal Fillers, and/or PRP with Microneedling.

RECEIVE HANDS-ON CERTIFICATE #2

Frequently Asked Questions: PracticalCME Botox and Dermal Filler Training in Charlotte / Mooresville

Clear answers on venue, format, faculty, curriculum, credit hours (including nursing pharmacology), scope of practice, and logistics. For the full positioning, see the Botox and Filler training hub page.

Where is the Charlotte training venue located?

The PracticalCME Charlotte clinical training site is located in Mooresville, North Carolina (Lake Norman area, approximately 30 miles north of downtown Charlotte), inside Dr. Andy Castillo and Dr. Rebecca Appleton Castillo’s shared practice. The venue is accessible from Charlotte Douglas International Airport (CLT) and serves aesthetic clinicians across North Carolina, South Carolina, and the broader Carolinas and Southeast. The site is a dedicated clinical facility, not a hotel conference room.

What is the live hands-on day like at the Charlotte / Mooresville session?

The hands-on day runs 4.5 hours. The time is spent on a brief review and Q&A, patient assessment, treatment planning for each patient on the day’s schedule, and hands-on injection on live patients under direct MD supervision. Both Dr. Castillos co-teach the session, which gives attendees two licensed MDs in the room throughout the day. The accredited 7.25-hour online CME course is prerequisite and self-paced, so no part of the live Charlotte day is spent catching up on didactic content. Every attendee performs the full workflow: anesthetic, facial prep, injection, and aftercare.

Who teaches the Charlotte session?

Dr. Alissandro “Andy” Castillo, MD and Dr. Rebecca Appleton Castillo, MD, husband-and-wife co-directors of the PracticalCME Charlotte / Mooresville Clinical Training Site, personally lead the live hands-on together. Both are state-licensed physicians in North Carolina with extensive aesthetic injection experience. Having two MDs on-site is a differentiator relative to competitor training programs that run with a single instructor or non-physician assistants. The underlying Botox and Dermal Filler curriculum is authored and continuously revised by Dr. Gregory P. Zengo, MD, FACOG, Founder and Medical Director of PracticalCME, who was trained in botulinum toxin by Dr. Jean Carruthers in 2001.

How many hours of CME credit is the Charlotte Botox and Filler training?

11.75 total contact hours: 7.25 ACCME-accredited online CME hours (including 4.7 nursing pharmacology hours) plus a separate 4.5-hour Hands-On Certificate of Completion. Credit totals are identical at every PracticalCME city.

Does the Charlotte course count for nursing pharmacology hours?

Yes. 4.7 pharmacology hours are included within the 7.25 online CME hours for both AANP (nurse practitioners) and ANCC (registered nurses). This is only possible because PracticalCME holds direct ACCME accreditation through the Global Education Group joint provider arrangement. Programs that route accreditation through AAFP, dental boards, or state society equivalence credit cannot award pharmacology hours regardless of how the certificate is worded. Nurses who have struggled to meet pharmacology hours each licensing cycle find this a significant benefit. Rolled out in 2026 after direct attendee feedback.

What is covered in the Botox curriculum at the Charlotte session?

FDA-labeled indications: glabella, lateral canthal lines (crow’s feet), forehead, and platysma bands (Botox FDA-approved 2024). Dosing and handling for all 6 FDA-approved neurotoxin brands. Off-label indications well-supported in the peer-reviewed literature and included in both the online didactic and the live hands-on: masseter and lower face (DAO, mentalis), small-dose frontalis for brow balance, bunny lines, gummy smile, and low-volume reconstitution technique. Not taught by design: Trap Tox at supraphysiologic doses, Barbie Botox, and any technique whose evidence base is a viral video rather than a peer-reviewed study.

What is covered in the dermal filler curriculum?

Hands-on filler technique in Charlotte: lips (volume and shape), perioral lines, nasolabial folds, and marionette lines. Technique and safety instruction: needle versus cannula selection, vascular anatomy and danger zones, product selection by indication, hyaluronidase reversal, vascular occlusion protocol, and the step-by-step vision-loss response algorithm. Not taught by design: mid-face filler, chin and jaw projection with HA, and high-volume cheek sculpting. The decision to keep mid-face out of the curriculum is deliberate and based on 24 years of injection experience.

How much toxin and filler is included for hands-on practice?

Every Charlotte attendee receives a guaranteed 20 units of BTX-A and 1cc of hyaluronic acid filler for live injection practice at no additional charge. The product is included in tuition and dispensed on the day of the hands-on.

Can I bring my own patients for additional hands-on?

Yes. There is no cap on hands-on if you bring your own suitable patients and purchase additional toxin and filler at below-retail rates on the day of the Charlotte / Mooresville training. Many providers bring one or two additional patients to extend their supervised experience before performing procedures in their own practice.

How are the hands-on patients recruited and consented?

Every hands-on patient at the Charlotte training is consented by one of the Dr. Castillos as a PracticalCME patient. Attendees never pay the patient, never collect money from the patient, never sign a treatment agreement with the patient, and never carry individual liability for the hands-on treatment. The faculty MD is the treating physician on the record. PracticalCME does not use paid community models who charge the training company.

Which licensed providers can register for the Charlotte training?

Registered Nurses, Nurse Practitioners, Physician Assistants, Physicians (MD and DO), and Dentists (DDS/DMD) where their legal scope of practice allows aesthetic injection. Because the Charlotte metro area spans two states (North Carolina and South Carolina), attendees should confirm scope for the state where they plan to practice. The curriculum covers North Carolina and South Carolina delegation and supervising physician requirements in detail. Completing a training course does not alter the legal scope of practice for the attendee’s licensure.

Can an RN or NP from another state attend the Charlotte training on a compact license?

North Carolina and South Carolina are both RN compact license states, so RNs from any compact state can practice as RNs in both. However, an NP license is state-specific and does not travel with the compact. An NP from outside North Carolina attending the Charlotte training should confirm their own home-state licensure for aesthetic injection before offering the procedure in their own practice. Both Dr. Castillos are licensed in North Carolina and serve as the treating physicians on the record for all hands-on patients.

Is a payment plan available for the Charlotte training?

Yes. Affirm financing is available at checkout, with rates as low as 0% APR for 6 months for qualified applicants. Longer repayment terms are available at standard APR. Tuition and the Affirm option are published on the product page. There is no sales call to find out the price and no scholarship application process. The accredited online course is accessible immediately upon purchase, and the Charlotte hands-on component can be scheduled at any available date within the two-year access window.

Charlotte Botox Hands-On Courses for Nurses, Physicians and Nurse Practitioners are taught by PracticalCME throughout the year. PracticalCME’s FAST TRACK ™ Online plus Live Blended learning allows participants to learn the material at home at your own pace prior to the live hands-on training date. Therefore, you will be fully confident in your knowledge, and earn your first of two CME certificates before you even attend the live Charlotte class date. Then, at the live date you will experience…

Our blended dual-certification online + live training leads the industry. As a result, PracticalCME has earned an average of 4.8 stars in reviews from actual attendees since our first blended FAST TRACK Botox Training Course was held in 2014. In addition, we have paired up our Botox and Dermal Filler Training with Cosmetic Platelet-Rich Plasma (PRP) so that you can certify in both at the same live training date, because we know your time is valuable.

Why are PracticalCME Charlotte Trainees More Successful?

Our Botox Training Course focuses on customizing the treatment plan to the patient’s own movement, and never follows a “cookbook” approach. For example, we teach a different dilution and precise muscle identification techniques so you can truly isolate the true cause of the patient’s wrinkles and effectively stop the clock on their aging process even on your first few patients, without having to see the patient back for touch-up treatments. The online course material drills these techniques home with additional treatment videos demonstrating the best technique prior to your live hands-on training class.

Our Dermal Filler Training Course curriculum focuses on using less product but at the proper depth to achieve the most natural filling of static folds and wrinkles of the face, in addition to the most natural lip reinflation techniques. Moreover, you will be impressed with the results when simply using the right technique and depth at your supervised hands-on treatment session. Their folds and wrinkles will disappear before your eyes while your patient remains comfortable.

As a result, PracticalCME has heard from many RN’s who took our training and taught their supervising physicians a few things upon return to the office.

Space is limited, So Register Today and Begin Learning Tonight

Each live date is limited to 5 participants before it is marked SOLD OUT. In fact, PracticalCME has only around 100 live training seats in Botox and Dermal Filler Training annually. Our award-winning, all-MD faculty take time away from their own successful practices to teach only a few times a year. This is why so many people travel to experience our small-group, in-depth blended online + live certification. Therefore, if the next Charlotte live training is sold out, scroll down and register now for the next open date. Then, you will receive your online content and can still begin learning and earning your first certification tonight.

Charlotte, NC CME
PracticalCME Training Center
Mooresville, NC
Botox Certification
Live Charlotte CME Certification Botox Training Courses

SINCE 2008, PRACTICALCME HAS BEEN THE LEADER IN SMALL-GROUP LIVE TRAINING COURSES AND ONLINE VIDEO COURSES IN POPULAR AESTHETIC AND WELLNESS SERVICES OFFERING CME CREDIT AND CERTIFICATION TO HUNDREDS OF DOCTORS, NURSE PRACTITIONERS, PHYSICIAN ASSISTANTS, AND NURSES.

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